Study of a ball-burnishing vibration-assisted process

This study refers to the study of the ball-burnishing process assisted by vibration. This study begins by considering that this vibration helps to make the development of this finishing process easier because it helps to deform the workpiece material more easily. Because a similar tool is not available in the market, a tool that can perform the study needed to be designed, characterised and manufactured to conduct the study by considering the critical components that are involved in the design and the physical model that characterises the operation. For these criteria, the tool operation is also characterised by evaluating the surface roughness that remains after the process occurs. Workpieces of aluminium and steel were used for the experimental validation. These results were compared to those obtained using the same tool without vibration. The roughness results obtained using the ball-burnishing vibration-assisted process improve compared to those obtained using the process without assistance for both materials tested

Switches, Excitable Responses and Oscillations in the Ring1B/Bmi1 Ubiquitination System

In an active, self-ubiquitinated state, the Ring1B ligase monoubiquitinates histone H2A playing a critical role in Polycomb-mediated gene silencing. Following ubiquitination by external ligases, Ring1B is targeted for proteosomal degradation. Using biochemical data and computational modeling, we show that the Ring1B ligase can exhibit abrupt switches, overshoot transitions and self-perpetuating oscillations between its distinct ubiquitination and activity states. These different Ring1B states display canonical or multiply branched, atypical polyubiquitin chains and involve association with the Polycomb-group protein Bmi1. Bistable switches and oscillations may lead to all-or-none histone H2A monoubiquitination rates and result in discrete periods of gene (in)activity. Switches, overshoots and oscillations in Ring1B catalytic activity and proteosomal degradation are controlled by the abundances of Bmi1 and Ring1B, and the activities and abundances of external ligases and deubiquitinases, such as E6-AP and USP7